FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005181134

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005181134
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12991792
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGGTA65500.025.2779965
CAGTGGT71550.023.1407594
GTGGTAT98900.017.1779696
AGCAGTG112500.015.57692
GTATCAA110900.015.3207489
TGGTATC113600.015.0199867
GGTATCA117750.014.3683378
AAGCAGT133200.013.1000071
GCAGTGG128550.012.8242253
Produced by FastQC (version 0.11.5)