FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005181140

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005181140
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13172378
Sequences flagged as poor quality0
Sequence length150
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATACG58400.030.8303727
GTAATAC82300.022.3147476
ATCTGTA92400.020.8748112
GATCTGT95750.020.6009351
TCTGTAA92400.020.3295753
CTGTAAT91050.019.9986324
ATACGAC93750.019.744299
TGTAATA102500.017.6944245
GGACTAG6857.2759576E-1216.8108314
CATCTAG14650.015.241549545-49
GACTAGC7404.656613E-1014.5887915
AATACGA128700.014.4934868
CTAGCCT7651.1463271E-813.1800867
ACTAGCC7951.9634172E-812.6827246
CTCACTA30400.012.26336515-19
ATCTAGT19000.012.13113645-49
TCTAGTA21550.011.56468745-49
ACTATAG34850.011.27569515-19
CTATAGC36900.010.68827615-19
GACTCAC35650.010.58363110-14
Produced by FastQC (version 0.11.5)