FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005181141

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005181141
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13172378
Sequences flagged as poor quality0
Sequence length150
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCACTA31750.058.7662288
TTAACCC38050.049.93752
TCACTAA43550.043.505029
ATTAACC43850.043.343831
TAACCCT46350.040.5287633
CCTCACT56700.033.161127
AACCCTC65100.029.436464
ACCCTCA74350.025.7742255
CCCTCAC83100.023.493626
ACTAGCC8500.018.645996
GACTAGC9350.016.1805275
CTAGCCT9550.015.8411867
GGACTAG9250.015.576624
CTAGATG18200.012.42284855-59
ACTAGAT20750.011.65806150-54
TACTAGA22600.010.8948950-54
TTACTAG24900.09.77287950-54
CACTAAA43900.09.351006510-14
TAGTGAG33150.08.86188985-89
CTAAAGG46150.08.6766310-14
Produced by FastQC (version 0.11.5)