FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005181142

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005181142
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15580627
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGAGTCAAATCTCGTAT677870.4350723497841261TruSeq Adapter, Index 5 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA218850.053.980269
AGAGCAC233900.050.540188
GATCGGA256050.046.792731
TCGGAAG281900.042.3603323
TAGCATG51450.040.579488
ATCGGAA311600.038.7624742
AAGAGCA326150.036.7750137
GAAGAGC342300.035.06036
GGAAGAG368250.032.6877145
CGGAAGA390650.030.6596744
TAAGGGG47500.026.2195639
AGTGAGT50950.025.27661
GTGAGTA53450.023.7442842
CCTTTAG90650.022.431954
TTAGCAT108750.019.7941657
GAGTCAA130250.017.89283235-39
AGTCACA134550.017.5822925-29
AGAGTCA138800.016.93908530-34
CTTTAGC121700.016.9186825
TTTAGCA127750.016.8498946
Produced by FastQC (version 0.11.5)