FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005181143

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005181143
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15580627
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCTCTTCAGTGTAGATCT705550.4528380019623087Illumina Single End PCR Primer 1 (96% over 32bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA254850.046.5692181
AGAGCGT267050.043.837368
TCGGAAG283450.041.4798623
GAGCGTC294800.039.6620379
AAGAGCG295700.039.4682777
TAGCATG54150.039.355898
ATCGGAA310800.038.1540152
GAAGAGC352050.033.3144956
GGAAGAG369000.031.842735
TAAGGGG48650.029.7460759
CGGAAGA398850.029.6401234
AGTGAGT53250.027.520621
GTGAGTA56600.025.6958242
CCTTTAG86600.023.4448624
TTAGCAT105000.020.7078087
TTTAGCA122200.018.0287936
TAGATCT135250.017.72763440-44
CTTTAGC116800.017.5678425
AGTGTAG142350.016.53994640-44
CAGTGTA149300.015.88575635-39
Produced by FastQC (version 0.11.5)