FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005181147

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005181147
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11682834
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCTCAA91050.019.0570357
ATGGCTC104450.016.544115
CTCAAAC107150.016.326939
TGGCTCA110900.015.516976
GCTCAAA137300.012.8473578
TCTGATG147000.011.6601671
GATGGCT157100.011.2336784
CTCGGGT43250.08.45599420-24
CTGATGG216650.08.3828842
TGATGGC249200.07.66034843
CTCTAGG14450.06.576406550-54
GGTCCTC63500.06.23555245-49
GGGTGGG63850.06.1337325-29
CCTCTAG21150.05.786568650-54
AGGTCCT71250.05.63813245-49
TCGGGTG70750.05.454123525-29
AACACAG72650.05.29175910-14
ACACAGC77000.05.21720510-14
CAGGTCC82000.05.057018345-49
AGCGACC80350.05.01768415-19
Produced by FastQC (version 0.11.5)