FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005181152

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005181152
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17282691
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGTGGT108350.038.0034454
AGTGGTA117950.034.66595
AGCAGTG145200.028.4118962
GTGGTAT150500.027.2639756
GCAGTGG167050.024.7786253
TGGTATC181300.022.953097
GGTATCA186400.022.556838
AAGCAGT198500.021.4116361
GTATCAA196050.021.3730879
TTAGTCC49950.011.242699
TTTAGTC69250.08.837118
AACCCCT74050.08.5709392
ACCCCTT85000.07.79156543
CCTTTAG76950.07.7646226
TCTTGGG122550.07.04926935-39
GCAGAGT127800.06.883606415-19
CAACGCA135800.06.76473410-14
CTTTAGT110550.06.18694167
GGGGGGG94300.06.0420762
AACGCAG158950.05.9697310-14
Produced by FastQC (version 0.11.5)