FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005181153

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005181153
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17282691
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCTCAA121800.026.625757
TGGCTCA135250.024.3501016
ATGGCTC140900.023.2713415
CTCAAAC151700.022.3256539
GATGGCT174300.019.3895074
TCTGATG192550.017.3188781
GCTCAAA195650.017.2004038
CTGATGG251550.013.2069582
TGATGGC270300.012.5829553
CTCGGGT60100.010.60062920-24
TTAGTCC45800.010.519099
TTTAGTC64200.07.8403538
TCGGGTG90300.07.59685725-29
AGGTCCT96300.07.21385945-49
CCTTTAG74350.06.96344476
GGTCCTC102700.06.84833845-49
AACCCCT75800.06.7355692
ACACAGC117500.06.53588210-14
AACACAG117400.06.492449810-14
GGTGGGA111000.06.41335925-29
Produced by FastQC (version 0.11.5)