FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005181154

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005181154
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16688909
Sequences flagged as poor quality0
Sequence length150
%GC58

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGGTA150550.088.171795
GTATCAA177900.075.314939
CAGTGGT178750.074.3426064
GTGGTAT194300.068.688886
GGTATCA210500.063.7535638
TGGTATC223200.059.7066767
AGCAGTG231150.057.6282122
AAGCAGT244000.054.9576721
GCAGTGG268750.049.7147753
TATCAAC183900.015.11144710-14
TCTTGGG171750.014.92207835-39
GCAGAGT210350.013.07358115-19
CAACGCA218550.012.86713710-14
AACGCAG259000.010.80754410-14
ATCAACG294150.09.90278910-14
CAGAGTT94500.09.38539715-19
CTTGGGA107700.09.33137835-39
TCAACGC315850.09.24066810-14
CAGAGTA69550.09.08805815-19
ACGCAGA324300.08.83506615-19
Produced by FastQC (version 0.11.5)