FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005181155

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005181155
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16688909
Sequences flagged as poor quality0
Sequence length150
%GC58

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTC431940.2588185962305864No Hit
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTC296090.17741722961039574No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGGCTC188100.065.9801945
GGCTCAA195350.063.973767
TCTGATG200600.062.2397041
CTCAAAC202150.061.6431969
TGGCTCA209150.059.5465776
GATGGCT231450.053.8397454
GCTCAAA265550.046.980198
CTGATGG360700.034.7086452
TGATGGC422500.030.0045573
CCTCTAG33600.023.2857550-54
CTCTAGG22500.020.71062350-54
TCTAGGA23550.019.97043450-54
CTAGGAT24150.019.23605350-54
CTCGGGT130650.018.7566920-24
CTCTAGC20450.016.87907250-54
CTAGCAC20700.016.81417850-54
AACACAG157050.016.56503110-14
TCTAGCA22350.015.89461750-54
TCGGGTG165650.014.95004225-29
TGGGAAC164200.014.93323130-34
Produced by FastQC (version 0.11.5)