FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005181159

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005181159
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18400867
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCTCAA124500.023.1898987
TGGCTCA136900.021.0360326
ATGGCTC141800.020.6137525
CTCAAAC145250.020.2233739
GCTCAAA192700.015.3186748
TCTGATG191100.015.0897431
GATGGCT208900.014.0960694
CTGATGG296100.010.4352022
CTCGGGT58450.010.07530220-24
TGATGGC318000.010.0077543
CCTCTAG25600.06.97444350-54
TCGAGTG54500.06.60564143
CGAGTGT43700.06.4253434
TCGGGTG104400.06.096230525-29
AGGTCCT113000.05.6572245-49
GGTCCTC110850.05.6240745-49
AACACAG120650.05.58542210-14
AGCGACC116600.05.56934815-19
GGGTGGG117500.05.563621525-29
AACACGT87850.05.45807830-34
Produced by FastQC (version 0.11.5)