FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005181161

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005181161
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17372608
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGCTCA115000.016.7778196
ATGGCTC116350.016.5831455
GGCTCAA129850.014.9705947
CTCAAAC143200.013.8762089
TCTGATG141750.013.6499931
GATGGCT159700.012.532884
GCTCAAA215900.09.5707348
CCTCTAG13700.09.03882950-54
CTGATGG271850.07.68386552
TGATGGC302950.07.2014743
CTCGGGT57950.07.106096720-24
CTCTAGG10600.05.976969250-54
CTAGCAC12450.05.43579150-54
AGGTCCT91250.05.03336945-49
Produced by FastQC (version 0.11.5)