FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005181163

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005181163
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18621545
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACACA76600.017.6626745
ACACAAG85400.016.0974317
TACACAA85700.016.0390976
GGTACAC103850.013.7902344
AAGGTAC114450.012.5230142
AGGTACA121350.011.8616953
CTAACAC32600.08.69934455-59
CACAAGG176550.08.1532458
AAAGGTA193950.07.8542371
GCTAACA49400.06.7615150-54
TGCTAAC48050.06.741736450-54
ACAAGGC260200.05.97488839
GTTGCTA56800.05.627134350-54
ACACACT63800.05.05434955-59
Produced by FastQC (version 0.11.5)