FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005181169

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005181169
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13918263
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCTCAA115800.024.4965637
CTCAAAC147750.019.7348639
ATGGCTC163100.017.568355
GCTCAAA167350.016.9506538
TGGCTCA181250.015.6502076
TCTGATG222950.012.6448461
GATGGCT239250.012.3378214
CTGATGG279250.010.3686092
CTCGGGT55000.010.20984520-24
GATCTAG10105.403963E-79.9807143
TGATGGC345250.09.2807113
CCTCTAG18600.08.98001950-54
GGGTGGG75800.07.940651425-29
GGTCCTC80000.07.37898745-49
GGTGGGA88900.06.88392725-29
AGGTCCT89050.06.709915645-49
ACACAGC96900.06.64235210-14
TCGGGTG93700.06.577384525-29
AGCGACC102050.06.43394715-19
CCTCGGG96600.06.29002720-24
Produced by FastQC (version 0.11.5)