FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005181170

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005181170
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16832755
Sequences flagged as poor quality0
Sequence length150
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGTGGT100750.041.448544
AGTGGTA114550.036.4551735
AGCAGTG129550.032.6830752
GTGGTAT144950.029.157226
GCAGTGG151350.027.7350643
TGGTATC158500.026.7554517
AAGCAGT167500.025.162121
GTATCAA174000.024.4108339
GGTATCA172550.024.4073568
GCAGAGT105800.08.23356215-19
TCTTGGG113150.07.507920335-39
CAACGCA127600.07.199229210-14
AACGCAG136000.06.60635210-14
TATCAAC172100.05.56361110-14
CGCAGAG187150.05.270099615-19
TCAACGC194150.05.22838710-14
ACGCAGA190750.05.08760515-19
Produced by FastQC (version 0.11.5)