FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005181171

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005181171
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16832755
Sequences flagged as poor quality0
Sequence length150
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCAAAC122200.024.7439069
ATGGCTC120900.024.4173935
TGGCTCA126050.023.3626566
GGCTCAA139000.021.806047
TCTGATG137700.021.310511
GCTCAAA157600.019.5979468
GATGGCT157200.019.0624494
CTGATGG235150.012.9644112
CCTCTAG14700.012.43940450-54
TGATGGC284850.011.4814883
CTCTAGG10850.010.7490150-54
TCTAGGA13600.08.78723750-54
AACACAG84650.07.7225310-14
CTAGCAC10650.07.030187650-54
CTCGGGT93000.06.997956320-24
ACACAGC94100.06.977597710-14
CTAGGAT16650.06.918133350-54
CTAAGAG15802.130089E-46.3794098
CAAACAC113700.06.192686610-14
GTGGGAA107300.06.051954725-29
Produced by FastQC (version 0.11.5)