FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005181173

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005181173
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13577951
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCTCAA92200.017.6477017
TGGCTCA99400.016.1528196
CTCAAAC107500.015.0681219
ATGGCTC107150.014.9173145
GCTCAAA132800.012.3066558
TCTGATG139250.011.2149311
GATGGCT161550.010.2558084
CCTCTAG15700.08.25377250-54
CTGATGG197600.08.1698972
TGATGGC228200.07.5449313
CTCGGGT48550.07.028624520-24
CTCTAGG14250.06.56762150-54
GGTCCTC63350.05.59119745-49
GGGTGGG70250.05.308447425-29
CTAGGAT20800.05.12245350-54
ACACAGC77500.05.109190510-14
Produced by FastQC (version 0.11.5)