FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005181176

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005181176
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15211409
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTC353150.23216126790095515No Hit
AGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAG247620.16278570906876544No Hit
GAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGA160610.10558522224995726No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGTGGT205600.078.815854
AGTGGTA218750.074.043765
AGCAGTG230250.070.5300752
GCAGTGG257850.063.0125473
GTGGTAT258700.062.804276
AAGCAGT270300.059.6328741
TGGTATC274750.059.1952487
GGTATCA278300.058.8281948
GTATCAA299750.054.594459
TCTTGGG199900.015.79552835-39
GCAGAGT215650.015.11560615-19
AACGCAG227100.014.77925710-14
CAACGCA230050.014.77750810-14
TCAACGC250600.013.61741710-14
ACGCAGA255750.013.08335415-19
CGCAGAG263950.012.63326215-19
ATCAACG276850.012.31585710-14
CTTGGGG134650.012.10454235-39
TATCAAC295000.011.6215710-14
CAGAGTT115400.09.10784515-19
Produced by FastQC (version 0.11.5)