FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005181177

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005181177
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15211409
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTC527040.34647677937001103No Hit
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTC365120.24003036142148304No Hit
AGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAG362790.23849861640036105No Hit
GAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGA255790.16815667766214162No Hit
AAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAA180530.1186806560786052No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCTCAA191850.076.27
CTCAAAC204200.071.659979
ATGGCTC204750.071.36335
TGGCTCA207150.070.8148966
GCTCAAA231850.063.083968
TCTGATG247100.059.5157851
GATGGCT250650.058.294784
CTGATGG319800.046.500912
TGATGGC321250.046.1774333
CTCGGGT131150.021.75578520-24
CCTCTAG47200.020.81176650-54
CTCTAGG33100.019.16250-54
GGTCCTC158200.018.15612845-49
AGGTCCT166050.017.30646145-49
AGCGACC178300.016.58322115-19
GGGTGGG179000.016.25379625-29
TCGGGTG177350.016.2428325-29
CCTCGGG182750.016.1166220-24
TCTAGGA38950.016.02553650-54
CAGGTCC182600.015.85603645-49
Produced by FastQC (version 0.11.5)