FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005181180

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005181180
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16521535
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGTGGT130500.054.1718754
AGTGGTA131150.053.793615
AGCAGTG171600.041.5613142
GTGGTAT172700.041.0181126
GTATCAA189150.037.8314449
GCAGTGG191400.036.8225333
GGTATCA195250.036.575788
TGGTATC200650.035.555557
AAGCAGT212400.033.412261
GAGCACA171900.015.1602239
AGAGCAC178500.014.4786858
GATCGGA185800.014.2205571
TCGGAAG206350.012.559463
ATCGGAA250700.010.8907122
TCTTGGG136600.010.22466835-39
AAGAGCA262850.010.1062787
GCAGAGT157750.09.44665515-19
CAACGCA162750.09.41290210-14
GAAGAGC312550.08.6604656
GGAAGAG314400.08.3805295
Produced by FastQC (version 0.11.5)