FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005181181

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005181181
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16521535
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTC212230.12845658711493818No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCTCAA152800.035.1978847
ATGGCTC155950.033.932825
TGGCTCA164700.032.916936
CTCAAAC166900.032.6980329
TCTGATG189700.027.268771
GATGGCT201450.026.3762844
GCTCAAA211400.025.4409648
CTGATGG297600.018.2502752
TGATGGC341700.016.4571863
GATCGGA191050.013.824461
CTCGGGT82750.013.06754120-24
CCTCTAG26150.012.27892550-54
TCGGAAG213400.012.0454583
AGAGCGT221100.011.7553738
GAGCGTC240650.010.8001589
ATCGGAA251100.010.6715432
AAGAGCG260200.09.7122347
AACACAG122300.09.45421510-14
CTCTAGG20750.09.29852150-54
TCGGGTG123000.09.29516925-29
Produced by FastQC (version 0.11.5)