FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005181194

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005181194
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11424156
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGTGGT72850.048.428274
AGTGGTA91450.038.6571855
AGCAGTG99550.036.094672
GCAGTGG107900.032.697943
GTGGTAT115200.031.1874966
TGGTATC122400.029.2941157
AAGCAGT136500.026.9077261
GGTATCA140850.025.6077548
GTATCAA150250.024.2931659
GCAGAGT82500.09.25017915-19
CAACGCA100750.07.93184610-14
TCTTGGG98500.07.61601535-39
CGCAGAG108550.07.242542315-19
AACGCAG109650.07.222380610-14
ACGCAGA124500.06.69633915-19
TCAACGC131150.06.323834410-14
CCCTTAA33557.6397555E-116.2235464
TATCAAC141200.05.95531110-14
CCACTCA38306.184564E-115.82711469
CAGAGTC37050.05.71290915-19
Produced by FastQC (version 0.11.5)