FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005181205

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005181205
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12944988
Sequences flagged as poor quality0
Sequence length150
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCAAAC94800.019.8216199
TGGCTCA92650.019.7378716
GGCTCAA93450.019.2608017
ATGGCTC98950.018.9905135
TCTGATG110550.016.6405181
GCTCAAA133050.013.9610098
GATGGCT140100.013.46454
CTCTAGG7250.010.52608250-54
CTGATGG212850.09.4409442
CCTCTAG13600.09.422850-54
CTAGGAT9300.08.5154750-54
TGATGGC249950.08.4117623
CTCGGGT50850.07.730214620-24
TCTAGGA10550.07.506528450-54
CTAGCAC7400.07.00486450-54
AACACAG67250.06.35904210-14
CCACTCA34301.364242E-106.08710539
ACACAGC81050.05.59609510-14
GGGTGGG76850.05.50859425-29
GGTCCTC85250.05.117419245-49
Produced by FastQC (version 0.11.5)