FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005181206

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005181206
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16285550
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGGCCGATTTCCCCCCGCCCGTTCCGAACGATTTCGCTATCTCACGCCG246700.15148398426826234No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGGTA125150.043.8324285
GTGGTAT154550.035.4476176
CAGTGGT158350.035.0061264
AGCAGTG177900.031.1415752
GGTATCA178600.031.0370668
GTATCAA190200.029.409119
TGGTATC190000.029.212737
AGGGCCG134500.028.2634931
GGGCCGA136950.027.7755132
AAGCAGT221350.025.4318281
GCCGATT177000.021.9223384
GGCCGAT184300.021.0149483
GCAGTGG297150.018.9211123
CCGATTT207350.018.6093885
TTTCCCC209050.018.2170019
GCTAGCG41000.018.191717115-119
CTAGCGA40900.017.98976115-119
ATTTCCC215400.017.9807578
GGCTAGC54900.013.952983115-119
GATTTCC301000.013.2738797
Produced by FastQC (version 0.11.5)