FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005181207

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005181207
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16285550
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGGCCGATTTCCCCCCGCCCGTTCCGAACGATTTCGCTATCTCACGCCG216180.13274344434176308No Hit
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTC169070.10381595954696034No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGGCTC138800.031.2779065
GGCTCAA161950.027.206817
GATGGCT168100.025.9977724
AGGGCCG137350.025.4608961
GGGCCGA136100.025.351092
CTCAAAC181400.024.1303799
TCTGATG190900.022.6785661
GCTCAAA191800.022.634558
GCCGATT171550.020.060994
GGCCGAT182750.018.7148383
CTGATGG239250.018.4856912
TGATGGC251900.017.8935283
CCGATTT198650.017.2515545
TTTCCCC203850.017.058249
CTAGCGA38550.016.99472115-119
GCTAGCG39550.016.710646115-119
ATTTCCC215600.016.128788
TGGCTCA278450.016.1083346
GGCTAGC52950.012.835212115-119
GATTTCC286350.012.2695827
Produced by FastQC (version 0.11.5)