FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005181209

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005181209
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14502068
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCATAGCCGGTGTAGATCT1011820.6977073890427213Illumina Single End PCR Primer 1 (96% over 32bp)
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTC535620.3693404278617367No Hit
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTC369170.2545636939504076No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCTCAA213650.073.576077
ATGGCTC224700.069.573345
CTCAAAC233350.068.0418559
TGGCTCA262950.059.7267466
GCTCAAA269250.058.7025388
TCTGATG277350.056.391331
GATGGCT277250.056.360274
GATCGGA313550.054.1536371
GAGCGTC340800.049.1633229
AGAGCGT342200.048.8991438
TCGGAAG352350.048.123193
CTGATGG329550.047.788582
AAGAGCG375900.045.0322347
ATCGGAA386800.044.229222
TGATGGC367700.043.0634273
GAAGAGC435700.039.0330926
CGGAAGA453150.037.608954
GGAAGAG463400.036.932385
CTCGGGT143000.021.48250220-24
CCTCTAG51950.018.90928850-54
Produced by FastQC (version 0.11.5)