FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005181211

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005181211
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13075411
Sequences flagged as poor quality0
Sequence length150
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCACTA29150.076.853448
TCACTAA36000.063.0303279
TTAACCC36600.060.7649772
ATTAACC42900.052.5651781
TAACCCT42950.052.2836273
CCTCACT58700.038.655737
CTAGCCT8200.036.89587
AACCCTC63050.035.760544
ACTAGCC9300.032.5319066
GGACTAG10500.030.1861844
GACTAGC10400.029.7837895
ACCCTCA79100.028.504455
CCCTCAC84300.027.3442216
ACTAGAT15450.015.75100850-54
CTAGATG15050.015.59715655-59
TACTAGA15750.015.45098850-54
TTACTAG15650.015.36569850-54
CACTAAA36800.012.64182810-14
CTAAAGG40700.011.501224510-14
ACTAAAG41450.011.43211310-14
Produced by FastQC (version 0.11.5)