FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005181220

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005181220
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13066527
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACTATA43000.020.09139
AACACTA45250.019.7280147
ACACTAT49750.018.6677448
CAAACAC81500.011.5713925
AAACACT78100.011.2455516
GCCCTTA47850.010.6823454
ACCAAAC90750.010.5564933
CCAAACA92850.010.0022574
TAGCCCT62450.07.8498932
TATTTAG71300.07.3710369
TACCAAA146200.06.5583042
CCCTTAT92350.06.08036475
ATACCAA172050.05.7927771
TTAGGCA39700.05.657705360-64
AGCCCTT98850.05.61085033
GTAGCCC98850.05.4459791
CCCCTCA44150.05.381269585-89
TACACTC45150.05.1027822115-119
Produced by FastQC (version 0.11.5)