FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005181225

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005181225
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16666351
Sequences flagged as poor quality0
Sequence length150
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTC940480.5642986878171472No Hit
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTC649160.38950337719396405No Hit
AGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAG297940.17876738585428806No Hit
GAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGA220580.13235050671859724No Hit
AAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAA168060.10083790987001294No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGATG292550.093.754181
ATGGCTC303500.090.50665
GGCTCAA305900.090.197217
CTCAAAC312700.088.005039
GCTCAAA336900.081.427618
GATGGCT341300.080.29314
TGGCTCA391250.070.851796
CTGATGG421800.065.176972
TGATGGC461000.059.85053
ATCCAAC86900.024.1626116
CCTCTAG74200.023.27920350-54
CTCGGGT245850.021.97154820-24
TCCAACT96900.021.8916767
CTCTAGG49250.021.40556750-54
CCAACTC100950.021.2982088
AGGTCCT257050.021.06654545-49
AACACAG269400.020.87835710-14
GGTCCTC260100.020.79186645-49
CAACTCT101000.020.7180339
TGGGAAC271350.019.9656130-34
Produced by FastQC (version 0.11.5)