FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005181234

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005181234
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16297614
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATACG90700.033.5076267
GATCTGT108650.027.9613781
GACTAGC9800.023.5014385
ATACGAC135000.022.6198259
GTAATAC150750.020.4467816
CTGTAAT157550.019.826254
GGACTAG11700.019.0697544
CAGTGGT72150.018.0555824
ATCTGTA174550.017.9777722
TCTGTAA175700.017.8601063
TGTAATA188750.016.3965345
AATACGA200900.015.5936528
ACTAGCC14500.015.3968616
CTAGCCT14900.014.5001837
AGTGGTA88700.014.3621755
GACTCAC45150.013.81049610-14
CGACTCA45650.013.40686710-14
AGCAGTG104400.013.23640252
CTCACTA46850.012.93495215-19
GCAGTGG114350.012.2105383
Produced by FastQC (version 0.11.5)