FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005181242

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005181242
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12248200
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGTGGT67500.029.6534584
AGTGGTA78600.026.2901865
AGCAGTG98750.021.291782
GTGGTAT105900.020.0567426
GCAGTGG112350.018.5850873
GTATCAA135750.015.5389389
TGGTATC142900.015.0651447
AAGCAGT145750.015.029581
GGTATCA150050.014.1060338
CCACTCA40950.012.6582059
GCCACTC57000.09.5991398
AGCCACT75700.07.8943537
AAGCCAC85700.06.80516246
ACGTTAA30051.540684E-96.22964334
ATACAAA161550.05.2359041
CAACGCA91700.05.213156710-14
TACAAAG118200.05.17806772
GCAGAGT92450.05.12417415-19
Produced by FastQC (version 0.11.5)