FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005181243

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005181243
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12248200
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCTCAA79900.017.1203427
TGGCTCA80300.016.6769036
ATGGCTC88250.015.5824835
CTCAAAC97450.014.9232879
CCACTCA40300.014.2915699
TCTGATG113750.011.9372621
GCCACTC52650.011.4865158
GCTCAAA134450.011.03097258
GATGGCT125700.010.7156284
AGCCACT74850.08.5605937
AAGCCAC80400.07.5221376
CTGATGG191850.07.2868712
CTCGGGT39200.07.16254720-24
TGATGGC206350.06.8070543
TACAAAG120800.05.96532632
ACGTTAA30451.33004505E-85.9137644
ATACAAA162750.05.5770371
CTCACTA24000.05.51962715-19
GTTACGT44153.8744474E-105.2630051
ACAAAGC139700.05.1046723
Produced by FastQC (version 0.11.5)