FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005181247

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005181247
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17817331
Sequences flagged as poor quality0
Sequence length150
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGCTCA128200.016.7149226
CTCAAAC130550.016.5239269
ATGGCTC128350.016.4711515
TCTGATG132500.016.0518991
GGCTCAA139600.015.813547
GATGGCT172750.012.9036834
GCTCAAA177300.012.2075998
CCTCTAG11650.011.85168850-54
CTCTAGG8350.010.50698950-54
TCTAGGA10350.08.61561450-54
CTGATGG261450.08.3337622
CTAGGAT10500.08.21858150-54
TGATGGC320400.07.2490333
CTAGCAC8900.06.787255350-54
AACACAG86600.05.646281710-14
CTCGGGT83000.05.47539420-24
CTCTAGC9851.8189894E-125.402570250-54
Produced by FastQC (version 0.11.5)