FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005181250

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005181250
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14131633
Sequences flagged as poor quality0
Sequence length150
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGGTA62050.031.0916235
CAGTGGT62150.030.1151944
GTGGTAT92950.020.6781776
AGCAGTG96300.019.6209722
TGGTATC105950.018.0753357
GGTATCA111700.017.4026878
GTATCAA111750.017.2660489
GCAGTGG112450.017.0926693
AAGCAGT126700.014.7995971
GATCTAA9251.949093E-59.3921771
CCTAAAC18305.5129567E-77.1211181
CCTAATT22102.645429E-76.55185751
CCTAACA16806.285768E-56.464111
CCTCTAA23451.8578245E-45.24847131
GGGGGGT22305.151154E-45.1944771
GGTAACA51759.094947E-125.1762841
CCTCTAC113300.05.1119531
GCAGAGT83700.05.074682715-19
TCTTGGG83700.05.07460735-39
Produced by FastQC (version 0.11.5)