FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005181268

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005181268
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16918568
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGGTA147600.052.043035
CAGTGGT151500.050.7571264
GTGGTAT190900.040.916466
AGCAGTG191450.040.615342
GCAGTGG211100.036.8020173
TGGTATC217700.035.8132977
GGTATCA231850.033.9070328
GTATCAA236500.033.3012439
AAGCAGT240150.032.3720471
TCTTGGG151300.010.2499435-39
CAACGCA171550.09.99560510-14
GCAGAGT173300.09.47929215-19
AACGCAG200200.08.34222710-14
TCAACGC210800.08.16861810-14
CGCAGAG220250.07.772392715-19
TATCAAC226000.07.64470710-14
ACGCAGA221500.07.605030515-19
ATCAACG247250.06.99350310-14
GGGGGGG43900.06.3961972
CTTGGGG112550.06.10263535-39
Produced by FastQC (version 0.11.5)