FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005181273

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005181273
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18856295
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCTCAA186400.020.467357
CTCAAAC192600.019.773479
GCTCAAA222000.017.4142518
TCTGATG231650.016.4319441
ATGGCTC231200.016.408525
TGGCTCA256650.014.6406286
CCTCTAG19650.012.23695650-54
GATGGCT325650.011.8707754
CTGATGG363850.011.0227522
CTCTAGG16500.09.24999250-54
CTAGCAC14900.08.31058650-54
CTCGGGT91800.08.28128220-24
TGATGGC507800.08.1372433
TCTAGGA17700.07.890743350-54
CTAGGAT20450.07.040867350-54
GGGTGGG124750.06.486207525-29
GGTCCTC124600.06.401540345-49
AACACAG136550.06.389982710-14
TGGGAAC133000.06.23603130-34
AGGTCCT137300.05.92475945-49
Produced by FastQC (version 0.11.5)