FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005181274

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005181274
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15829846
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGGTA80250.028.9742585
CAGTGGT91550.025.7128224
GTGGTAT127700.018.7155276
AGCAGTG136950.017.906672
GTATCAA136350.017.4771889
GGTATCA151350.016.030478
GCAGTGG153250.015.6894483
TGGTATC154150.015.645887
AAGCAGT177400.014.2448561
TCTTGGG99850.05.320946735-39
Produced by FastQC (version 0.11.5)