FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005181276

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005181276
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15476911
Sequences flagged as poor quality0
Sequence length150
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATACG59500.035.8280647
GTAATAC85150.024.9508886
TCTGTAA100450.022.0682893
CTGTAAT100900.021.8272084
TGTAATA101550.021.6166925
ATCTGTA105050.020.896412
GATCTGT118150.018.949591
ATCTAGT10950.018.94588545-49
GGACTAG4203.1146556E-818.8499984
TCTAGTA10950.018.68274945-49
ATACGAC121350.018.0427829
CATCTAG11500.018.03977845-49
CTAGTAA11850.017.1422345-49
ACTAGCC5151.7331331E-816.7811876
GACTAGC5002.2785389E-715.8340495
AATACGA152750.014.5217238
ACTATAG31750.014.05410715-19
CTCACTA33300.012.79477815-19
CTAGCCT6855.6822864E-712.6165117
CTATAGC39250.011.44195515-19
Produced by FastQC (version 0.11.5)