FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005181280

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005181280
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14419879
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCTGTAATACGACTCACTATAGCCGTTTCAGGCTTAATTAAACCATCT174360.12091640990884873No Hit
TCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTC156460.10850299090581829No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATACG84600.061.8003777
GGACTAG11700.061.5118524
GACTAGC14050.050.1989255
GTAATAC114200.046.097376
ATACGAC118350.044.2403079
TGTAATA123400.042.6914185
ACTAGCC17000.041.5147136
TCTGTAA138000.038.331243
CTAGCCT19250.037.4105577
ATCTGTA144200.036.9826132
GATCTGT145150.036.5981641
CTGTAAT149350.035.4182244
GCCTAAT26700.033.6991351
AATACGA160000.033.0820358
CTAATTG30000.030.7067183
AGTGGTA77650.025.7660625
CCTAATT37500.024.1815412
CAGTGGT86850.023.1195344
CATCTAG29900.019.65797645-49
TCTAGTA30000.019.64046945-49
Produced by FastQC (version 0.11.5)