FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005181285

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005181285
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15165564
Sequences flagged as poor quality0
Sequence length150
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAAACG27300.015.5600464
ACGCTAA29050.014.8705368
TCTAAAC29400.014.6940883
CCTCTAA31000.013.9713821
CGCTAAA43950.010.4843779
CTCTAAA42700.010.4544822
AACGCTA53200.08.3907667
TAAACGC73650.06.54973275
GATCGGA132200.06.11556241
GATTAAA72850.05.0534791
Produced by FastQC (version 0.11.5)