FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005181292

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005181292
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12683635
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGTGGT89200.036.96784
AGTGGTA109850.030.6055665
AGCAGTG114500.029.3016052
GCAGTGG127450.025.986093
GTGGTAT142450.023.7014646
AAGCAGT155950.022.066661
TGGTATC157800.021.2134237
GGTATCA187000.018.285928
GTATCAA196500.017.4385019
CGCAGAG100550.07.316922715-19
GCAGAGT101650.06.926137415-19
TCTTGGG105650.06.678241335-39
AACGCAG115950.06.41956110-14
CAACGCA119050.06.397522410-14
TCAACGC122050.06.145899310-14
ACGCAGA126250.05.987114415-19
Produced by FastQC (version 0.11.5)