FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005181299

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005181299
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14882060
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGCTCA113450.019.4823156
GGCTCAA114550.019.4214557
ATGGCTC115850.019.2030055
CTCAAAC128250.017.514759
TCTGATG136500.016.2623861
GATGGCT158150.014.4768284
GCTCAAA175150.013.0716678
CCTCTAG11050.011.07599150-54
CTGATGG215800.011.0476552
TGATGGC248450.09.6794173
CTCTAGG9400.08.27164450-54
CTCGGGT58450.07.808984320-24
GGGTGGG75700.06.790568425-29
TCTAGGA13050.06.068454750-54
AACACAG82150.06.04709110-14
GGTCCTC86450.05.995673745-49
CTAGCAC11050.05.863759550-54
GGTGGGA93600.05.67654825-29
CTAGGAT13450.05.673871550-54
AGGTCCT87300.05.64043245-49
Produced by FastQC (version 0.11.5)