FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005181302

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005181302
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14192910
Sequences flagged as poor quality0
Sequence length150
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGTGGT94650.036.1273354
AGTGGTA96200.035.6200755
AGCAGTG120450.028.8258252
GTGGTAT133300.025.9763366
GTATCAA137450.025.4539099
GCAGTGG143150.024.239223
GGTATCA153000.023.1021848
AAGCAGT152550.023.0080761
TGGTATC155700.022.1005067
TTAGACC15805.1593815E-88.2012197
TCTTGGG91550.07.738115335-39
CAACGCA111000.06.93942910-14
GTTAGAC19551.8046376E-66.62809476
TAGTTAG21802.2887252E-76.6044474
GCAGAGT114500.06.563914315-19
AACGCAG134550.05.46802110-14
CTAAGAG20057.5602403E-45.3856718
TATCAAC153850.05.324854410-14
TAGACCC29851.9461604E-65.3056838
Produced by FastQC (version 0.11.5)