FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005181303

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005181303
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14192910
Sequences flagged as poor quality0
Sequence length150
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCTCAA112450.023.3695457
CTCAAAC115800.022.0087939
ATGGCTC119200.021.6233065
TGGCTCA133800.019.3176216
GCTCAAA148500.017.744698
TCTGATG158900.016.0808221
GATGGCT177500.014.72423654
CTGATGG262000.09.86887552
TTAGACC16159.094947E-129.8076967
TGATGGC300800.09.2158083
CCTCTAG16150.09.09401950-54
AACACAG76450.07.778592610-14
GTTAGAC18951.7535058E-87.5986446
CTCGGGT70050.07.56410320-24
TAGTTAG21903.405694E-86.9039824
ACACAGC84650.06.650867510-14
CTCTAGG11600.06.5787950-54
GGGTGGG87600.06.492733525-29
GGTGGGA90200.06.289618525-29
CTAGGAT14800.06.129245350-54
Produced by FastQC (version 0.11.5)