FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005181304

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005181304
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13040889
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATACG84700.025.6800947
GATCTGT86300.024.9408861
GTAATAC101700.021.3874516
ATACGAC107300.020.4744159
CTGTAAT116950.018.7688584
TCTGTAA117150.018.4296553
ATCTGTA121750.017.6742292
TGTAATA127650.017.0829055
GGACTAG13450.016.0522984
CTAGCCT13350.015.6455137
GACTAGC14300.015.0981985
ACTAGCC15150.013.7866396
AATACGA166150.013.7414318
CATCTAG18750.012.83138745-49
ACTATAG39250.011.36797815-19
TCTAGTA23250.011.0294945-49
ATCTAGT23150.010.76597845-49
CTCACTA43150.010.17373215-19
CGACTCA45400.09.99221510-14
GACTCAC45800.09.87350310-14
Produced by FastQC (version 0.11.5)