FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005181306

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005181306
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11056201
Sequences flagged as poor quality0
Sequence length150
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCTACT38300.024.8111926
TCCCTAC47500.020.460335
GCTCCCT58900.017.732053
TGCTCCC60400.016.3513472
CTTAGTC65300.015.4350128
CTCCCTA69750.015.0692934
TTAGTCC73050.014.4873599
CCTTAGT76700.014.0790797
CTACCTC78650.013.4138711
CCTACTT105400.010.1087977
CTCCTTA113300.09.5944375
TACCTCC115350.09.4368982
TCCTTAG111600.09.3535456
ACCTCCT117600.09.0036013
ATGCTCC114150.08.8597961
CCCCCCA26250.08.28263610-14
CCTCCTT133400.08.0410854
GTCCCCC29300.07.86272910-14
TCCCCCC29400.07.640084310-14
CCAACCC40901.8189894E-125.9847739
Produced by FastQC (version 0.11.5)