FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005181316

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005181316
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14635078
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTC204110.1394662877778991No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGTGGT112150.048.0201574
AGTGGTA120900.044.6638535
AGCAGTG135850.039.912192
GTGGTAT156500.034.733926
GCAGTGG159150.033.9751973
TGGTATC178400.030.5507777
AAGCAGT178900.030.3024331
GTATCAA191300.028.9400969
GGTATCA190900.028.7744628
TCTTGGG124050.09.07691135-39
GCAGAGT129900.08.91197115-19
CAACGCA137200.08.75259310-14
AACGCAG146700.08.10727210-14
TCAACGC174000.07.06697310-14
CGCAGAG171200.07.0396115-19
ACGCAGA171600.06.989637415-19
TATCAAC185550.06.704673310-14
CTTGGGG91300.06.071808335-39
ATCAACG211050.06.065145510-14
GGGGGGG81450.05.657952
Produced by FastQC (version 0.11.5)