FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005181317

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005181317
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14635078
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCTCAA121050.035.2693987
TGGCTCA122900.033.803166
CTCAAAC126450.033.7046859
ATGGCTC125450.033.230845
TCTGATG152350.026.492831
GCTCAAA167050.025.7728758
GATGGCT173700.024.2592834
CTGATGG236350.017.7141022
TGATGGC253150.016.6179563
CCTCTAG19500.012.70011550-54
CTCGGGT75650.011.34363820-24
CTCTAGG15400.011.12602650-54
AGGTCCT96700.09.02329845-49
AACACAG100500.08.96884210-14
GGTCCTC98950.08.75991445-49
ACACAGC107100.08.6984710-14
TCTAGGA20800.08.16831650-54
CTAGCAC12000.08.15908650-54
CTAGGAT21250.08.06309650-54
GGGTGGG116300.07.86164125-29
Produced by FastQC (version 0.11.5)