FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005181322

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005181322
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17810395
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTC281560.15808745398403573No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGTGGT257050.079.2955864
AGTGGTA259000.078.6429755
GTGGTAT288000.070.624066
AGCAGTG303750.067.513132
GGTATCA310450.065.695828
TGGTATC310550.065.495837
GCAGTGG317100.064.370913
AAGCAGT327150.063.1553271
GTATCAA330700.061.847179
CTTAGTC41650.031.8042878
TTAGTCC46050.029.3907789
CTCCCTA50300.022.0434446
CCTTAGT74000.018.6808347
TCCTTAG81450.017.237346
TCTTGGG234350.017.05606335-39
GCAGAGT270850.015.36372315-19
CAACGCA279300.015.31635810-14
CCCTATT73600.015.1613098
TCCCTAT79800.014.255457
AACGCAG305200.013.80899310-14
Produced by FastQC (version 0.11.5)