FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005181324

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005181324
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16453206
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACTAT66500.019.1619688
AACACTA69800.018.1528857
CACTATA75350.017.5793679
TCGTGTG68300.015.0976443
ACCAAAC92850.014.5229063
AAACACT101550.013.25694756
CCAAACA115350.011.5024434
CAAACAC123250.011.1565765
TACCAAA129650.011.0125152
TTCGTGT100300.010.9997882
ATACCAA151150.09.909971
GTGTGCT109100.09.6341325
CGTGTGC118650.09.1161914
GTTCGTG144550.09.1109471
GATCGGA134850.07.6735321
TGTGCTT148550.07.56020936
AGAGCAC134800.07.1031378
CTCTAGG19452.0325024E-77.0687761
GAGCACA127300.06.89922149
CTATAGC47050.06.54865410-14
Produced by FastQC (version 0.11.5)